Fig. 5
From: Effects of novel HDAC inhibitors on urothelial carcinoma cells
Cellular effects of 19i on HDAC4-overexpressing UCCs. Cellular effects of 19i on VM-CUB1 and UM-UC-3 cell lines lentivirally transduced to overexpress HDAC4 compared to the parental cells and cell lines transduced with empty vector (LV). a Dose-response curves of UC cell lines treated with increasing concentrations of 19i (0.1–5 μM) for 72 h. Data shown are mean from n = 4. b Clonogenicity of cells treated with DMSO, 2 μM 19i, or 2.5 μM SAHA for 48 h. Note smaller colonies in VM-CUB1-HDAC4 DMSO-treated controls. Representative examples of triplicates. c Analysis of nuclear morphology, based on DAPI staining. The percentage amount of mitoses and micronuclei are shown after treatment with HDACi 19i (2 μM), SAHA (2.5 μM), or DMSO for 48 h. The calculated significances refer to the DMSO solvent control (*p < 0.05). Data shown are mean from n = 3