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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Effects of novel HDAC inhibitors on urothelial carcinoma cells

Fig. 3

Effects of 19i treatment on gene expression and protein acetylation in VM-CUB1. Effects on mRNA and protein expression levels in VM-CUB1 following treatment with 19i (2 μM), SAHA (2.5 μM), or DMSO as solvent control. a Expression of thymidylate synthase (TS) and p21CIP1(CDKN1A) mRNAs after 24 and 48 h of treatment as measured by qRT-PCR. b Acetylation of α-tubulin and histones H3 and H4 after 48 h of treatment with 2.5 μM SAHA, 1 or 2 μM 19i, or DMSO; ac acetylated. c HDAC4, HDAC5, HDAC7, and HDAC9 mRNA expression after 24 or 48 h of treatment. In a and c, all values indicate relative expression compared to a standard for each gene and adjusted to TBP as a reference gene. Significance levels refer to the DMSO solvent control (*p < 0.05). Data in a and c are mean from n = 3; the blot in b shows a representative experiment

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