From: Methylation of BRCA1 and MGMT genes in white blood cells are transmitted from mothers to daughters
 | Primers sequences | No. of CpG sites | Annealing temp |
---|---|---|---|
F1 R1Bio Sequencing | GGTATTGGATGTTTTTTTTTATAAGATTAT CCAATCCCCCACTCTTTC ATTATAGTTTTTAAGGAATATTGTG | 3 | 56 |
F2 R2 Sequencing | GAAAGAGTGGGGGATTGGGATT AAAATACCTACCCTCTAACCTCTACT ACCTCTACTCTTCCA | 4 | 60 |
F3 R3 Bio Sequencing | AGGGTAGGTATTTTATGGTAAATTTAGGT TATCTAAAAAACCCCACAACCTATCC ATGGTAAATTTAGGTAGAATTTT | 5 | 60 |
F4 R4Bio Sequencing | AGATTGGGTGGTTAATTTAGAGT TCTAAAAAACCCCACAACCTATCC GGAAAAGAGAGGGAATTATAGATAA | 6 | 58 |
F5 R5 Bio Sequencing | GGGGTAGATTGGGTGGTTAA TTATCTAAAAAACCCCACAACCTATC GAGAGGTTGTTGTTTAG | 5 | 58 |
BRCA1 | F 5′–TGTAGGCTCCTTTTGGTTATATCATTC–3′ R 5′–CATGCTGAAACT TCTCAACCAGAA–3′ | 59 °C | |
β- Actin | F 5′–TCC CTG GAG AAG AGC TAC GA–3′ R 5′–TGA AGG TAG TTT CGT GGA TGC–3′ | 59 °C |