Skip to main content

Table 4 Bisulfite pyrosequencing and real-time PCR primers

From: Methylation of BRCA1 and MGMT genes in white blood cells are transmitted from mothers to daughters

 

Primers sequences

No. of CpG sites

Annealing temp

F1

R1Bio

Sequencing

GGTATTGGATGTTTTTTTTTATAAGATTAT

CCAATCCCCCACTCTTTC

ATTATAGTTTTTAAGGAATATTGTG

3

56

F2

R2

Sequencing

GAAAGAGTGGGGGATTGGGATT

AAAATACCTACCCTCTAACCTCTACT

ACCTCTACTCTTCCA

4

60

F3

R3 Bio

Sequencing

AGGGTAGGTATTTTATGGTAAATTTAGGT

TATCTAAAAAACCCCACAACCTATCC

ATGGTAAATTTAGGTAGAATTTT

5

60

F4

R4Bio

Sequencing

AGATTGGGTGGTTAATTTAGAGT

TCTAAAAAACCCCACAACCTATCC

GGAAAAGAGAGGGAATTATAGATAA

6

58

F5

R5 Bio

Sequencing

GGGGTAGATTGGGTGGTTAA

TTATCTAAAAAACCCCACAACCTATC

GAGAGGTTGTTGTTTAG

5

58

BRCA1

F 5′–TGTAGGCTCCTTTTGGTTATATCATTC–3′

R 5′–CATGCTGAAACT TCTCAACCAGAA–3′

59 °C

β- Actin

F 5′–TCC CTG GAG AAG AGC TAC GA–3′

R 5′–TGA AGG TAG TTT CGT GGA TGC–3′

59 °C

  1. F forward, R reverse