Table 3 Primer pairs for real-time PCR
From: Vitamin C increases 5-hydroxymethylcytosine level and inhibits the growth of bladder cancer
| Genes | Sequence (5′ to 3′) | |
|---|---|---|
| GAPDH | Sense | GGTGAAGGTCGGAGTCAACG |
| Antisense | TGGGTGGAATCATATTGGAACA | |
| TET1 | Sense | AATGGAAGCACTGTGGTTTG |
| Antisense | ACATGGAGCTGCTCATCTTG | |
| TET2 | Sense | AATGGCAGCACATTGGTATG |
| Antisense | AGCTTCCACACTCCCAAACT | |
| TET3 | Sense | GAGGAGCGGTATGGAGAGAA |
| Antisense | AGTAGCTTCTCCTCCAGCGT | |
| SVCT1 | Sense | TCATCCTCCTCTCCCAGTACCT |
| Antisense | AGAGCAGCCACACGGTCAT | |
| SVCT2 | Sense | TCTTTGTGCTTGGATTTTCGAT |
| Antisense | ACGTTCAACACTTGATCGATTC | |
| IDH1 | Sense | TCCGTCACTTGGTGTGTAGG |
| Antisense | GGCTTGTGAGTGGATGGGTA | |
| IDH2 | Sense | TGAACTGCCAGATAATACGGG |
| Antisense | CTGACAGCCCCCACCTC | |
| L2HGDH | Sense | TCAAAAATTCATCCCTGAAATTACT |
| Antisense | CTACCAGATTTCCATCTCTATCCAG | |