Fig. 4

Vitamin C treatment increases 5hmC levels and decreases malignant phenotypes in T24 cells in vivo. a Tumor growth curves of xenografts with T24 cells treated with vitamin C or placebo. Tumor volume is shown as the mean ± SD (n = 8 mice). b Tumor weights of the indicated xenografts at the endpoint (39 days) are shown as the mean ± SD (n = 8 mice). Statistical significance was determined by the Mann-Whitney U test. c Images of xenograft tissues in vitamin C in treated and control groups (n = 8). d The body weights of mice during treatment (n = 8 mice). e 5hmC score and representative IHC staining of xenograft tissues. Scale bar, 50 μm. Statistical significance was determined by the Mann-Whitney U test