Fig. 3

Distinct methylome alterations occur in sperm of post-sepsis animals. a Circos plot depicting the distribution of differentially methylated cytosines (n = 381) over the chromosomes (orange: hypermethylated CLP vs. sham; blue: hypomethylated CLP vs. sham). b Distribution of differentially methylated cytosines according to genomic region. c Principal component analysis using the 381 cytosines for clustering. d Heat map representation of differential cytosine methylation after unsupervised hierarchical clustering separated into genomic regions (promoter, exons, intron); bottom annotation represents individual animal (C = CLP, S = sham). e–g Corresponding gene lists were separately subjected to gene ontology term analysis. Top five overrepresented biological processes are shown. Dashed red line depicts statistical threshold of p = 0.01. Data is derived from n = 5 animals of each experimental condition (sham or CLP)