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Fig. 5 | Clinical Epigenetics

Fig. 5

From: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer

Fig. 5

Analyses of distribution of H3K9me3 and H3K27me3 across the promoters of immune checkpoints in tumor and normal tissues. Cells from two individual NT and TT samples were isolated by enzyme disaggregation. Chromatin was precipitated using H3K9me3, H3K27me3 antibodies, and control IgG as negative control. Subsequent qPCR was performed using PD-1, CTLA-4, TIM-3, and LAG-3 promoter primers and data were normalized to input. ChIP analysis of distribution of H3K9me3 and H3K27me3 at PD-1 (a), CTLA-4 (b), TIM-3 (c), and LAG-3 (d) promoter regions are shown

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Clinical Epigenetics

ISSN: 1868-7083

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