Fig. 3

Analyses of CpG methylation status of PD-L1 and TIGIT promoters. CpG methylation status of the promoter regions of PD-L1 (a) and TIGIT (c) were analyzed by bisulfite sequencing of the genomic DNA isolated from the breast tumor and normal tissues. Representative plots from eight individual tumor and normal tissues show the methylation status of CpG motifs. Methylation status of individual CpG motifs is shown by white (demethylation) or gray (methylation). The bar charts show the demethylation percentage of PD-L1 (b) and TIGIT (d) from eight different NT and TT samples