Fig. 1

The dynamic ranges and sensitivities of ddMSP and qMSP for quantification of DNA methylation. a In methylated DNA serially diluted in sputum DNA of a healthy nonsmoker, ddMSP can detect levels of methylated 3OST2 as low as 0.04% (LOQ = 0.04%). A negative template control (NTC) sample was also tested. R² = 0.966 shows excellent linear correlation between measured concentration of methylated DNA and expected percentage of methylation. b qMSP can detect methylated 3OST2 at 0.10% (LOQ = 0.10%) in the same diluted samples with R² of 0.935. c In 100% methylated DNA serially diluted into water, ddMSP can detect the smallest amount of methylated DNA at 156 pg/μL (156 pg/μL) with R² of 0.959. d qMSP can detect the smallest amount of methylated DNA at 156 pg/μL (156 pg/μL) with R2 of 0.937