Fig. 3

The effect of RAI2 on cell proliferation and apoptosis in colorectal cancer cells. a The effects of RAI2 on colony formation in RKO and LOVO cell lines before and after the restoration of RAI2 expression. The experiment was repeated three times (***P < 0.001). b Growth curves demonstrate the effect of RAI2 on cell proliferation as measured by the MTT assay for 72 h in RKO and LOVO cell lines before and after the restoration of RAI2 expression, and in DLD1 cell line, before and after knockdown of RAI2. The experiment was repeated three times (***P < 0.001, *P < 0.05). c Upper and middle: percentage of apoptotic cells before and after the restoration of RAI2 expression in RKO and LOVO cells under the treatment of STS (100 ng/ml for 24 h). Down: percentage of apoptotic cells and before and after knockdown of RAI2 in DLD1 cells under the treatment of STS (120 ng/ml for 24 h). The experiment was repeated three times (***P < 0.001). d Expression levels of RAI2, caspase-3, and cleaved caspase-3 were detected by Western blot before and after the restoration of RAI2 expression in RKO and LOVO cells and before and after knockdown of RAI2 in DLD1 cells. Actin was used as a control.