Fig. 4

Epigenetic age predictions based on three different aging signatures. a The clustered heatmap represents DNAm of 99 age-associated CpG sites of the epigenetic age predictor of Weidner et al. [14]. Black rectangles highlight DNAm patterns of CpGs that may reveal moderate epigenetic age acceleration in HuMice. b Three epigenetic aging predictors from Weidner et al. [14], Horvath [16], and Hannum et al. [15] were used to predict donor age in 23 cord blood samples (CB; GSE40799, GSE30870), 15 whole blood samples of 5 year-old donors (GSE83334), 16 whole blood samples of 21–32 year-old donors (GSE65638), 20 peripheral blood mononuclear cell samples of 89–103 year-old donors (GSE30870), and the five samples from humanized mice (HuMice; GSE103010). The age predictor of Weidner et al. was not trained for CB and underestimates CB and HuMice samples to negative ages. c Epigenetic age predictors of Hannum et al. [15] and Horvath [16] were applied on DNAm profiles of CB cells, BM of HuMice, and peripheral blood of 5-year-old individuals. **P < 0.01; ***P < 0.001 (two-tailed Student’s t test)