Epigenome-wide association study of total serum immunoglobulin E in children: a life course approach

Clinical Epigenetics

Table 4 Life course analysis— contribution of fetal and postnatal influences (FDR threshold < 0.05)—asthma pathway. For the prenatal analysis, results are expressed as the change in log(IgE) concentration per 1% increase in cord blood methylation value. While for the postnatal analysis, results are expressed as the change in log(IgE) concentration per 1% increase in Δ-DNAm methylation value

				Prenatal influences			Postnatal influences
CpG	CHR	MAPINFO	Gene	Estimate	P value	FDR	Estimate	P value	FDR
Life course analysis—postnatal influences (asthma pathway)
cg26787239	5	132,008,525	IL4	− 0.12	9.00E−02	6.91E−01	− 0.18	1.94E−05	0.020
cg01310029	3	3,152,374	IL5RA	− 0.09	7.48E−02	6.73E−01	− 0.18	3.55E−06	0.005
cg02970679	17	56,269,818	EPX	− 0.17	9.50E−04	2.86E−01	− 0.25	5.06E−23	7.95E−18
cg25173129	17	56,269,410	EPX	− 0.16	5.73E−03	4.22E−01	− 0.22	2.59E−08	9.80E−05
cg27469152	17	56,282,313	EPX	− 0.10	5.02E−02	6.32E−01	− 0.19	2.59E−07	6.53E−04
cg18421167	17	56,276,490	EPX	− 0.09	2.38E−01	8.07E−01	− 0.25	1.83E−06	0.003
cg08105265	17	56,274,480	EPX	− 0.04	6.46E−01	9.40E−01	− 0.22	1.55E−05	0.017
cg12819873	11	57,157,632	PRG2	− 0.12	8.55E−03	4.60E−01	− 0.23	1.21E−09	9.05E-06
cg15700636	11	57,156,050	PRG2	− 0.08	3.19E−02	5.85E−01	− 0.18	4.13E−08	1.46E−04
cg08295410	5	156,990,663	ADAM19	− 0.31	4.79E−03	4.09E−01	− 0.40	2.70E−05	0.026

Model adjusted for maternal [age at enrollment (continuous), smoking status (smoking during pregnancy/former/never), college graduate (yes/no), maternal atopy history (yes/no)], children [child’s sex (female/male), race (white/black/other), gestational age (continuous), age at blood drawn (continuous)], and the cell-type proxys using the first five ReFACTor components estimated from cord blood
Corresponding model: log(IgE) = β₀ + β₁CpG__cbi + β₂ΔCpGi + β₃X₃ + … + β_pX_p + η
Life course analysis—prenatal influence: association between cord blood methylation and mid-childhood IgE independent of postnatal changes in DNA methylation, which correspond to β₁CpG__cbi;
Life course analysis—postnatal influence: association between changes in postnatal DNA methylation from birth and mid-childhood IgE independent of baseline/cord blood DNA methylation, which correspond to ΔCpG_i

ISSN: 1868-7083