Fig. 1

H19 expression and methylation in AML. a H19 expression level detected by real-time quantitative PCR in controls and AML patients. b H19 unmethylation level detected by real-time quantitative unmethylation-specific PCR in controls and AML patients. c, d H19 methylation density detected by bisulfite sequencing in controls and AML patients, respectively. White cycle, unmethylated CpG dinucleotide; black cycle, methylated CpG dinucleotide. e The correlations between H19 expression and unmethylation. f Relationship between H19 expression and methylation of two different regions using The Cancer Genome Atlas (TCGA) data. Left: H19 expression, value in log2(x + 1) transformation, x is the RSEM value. Middle: H19 methylation in HM27K. Right: H19 methylation in HM450K. Red color: higher expression/methylation. Blue color: lower expression/methylation. White color: intermediate level of H19 expression/methylation. Gray color: no data. Each row represents H19 expression and H19 methylation level of the same patient; each line from right to left in middle and right figures represents the methylation level at different sites