Metabolic and inflammatory biomarkers are associated with epigenetic aging acceleration estimates in the GOLDN study

Table 3 Multivariable linear regression models that relate biomarkers (rows) to epigenetic age acceleration (covariate) using the Horvath calculator and the Hannum calculator

	Horvath age acceleration			Hannum age acceleration (EEAA)
Dependent variable	Beta*	SE	p	Beta#	SE	p
LDL mg/dL (0 h)	0.36	0.21	0.09	0.166	0.12	0.17
LDL mg/dL (6 h)	0.003	0.06	0.96	− 0.006	0.04	0.87
HDL mg/dL (0 h)	0.06	0.08	0.52	− 0.11	0.04	0.02
HDL mg/dL (6 h)	0.05	0.03	0.05	0.004	0.15	0.81
TC mg/dL (0 h)	0.44	0.25	0.08	0.091	0.14	0.52
TC mg/dL (6 h)	0.15	0.08	0.06	0.068	0.05	0.13
TG mg/dL (0 h)	0.001	0.004	0.75	0.002	0.002	0.30
TG mg/dL (6 h)	0.0005	0.002	0.84	0.004	0.001	0.02
IL6 (0 h)	− 0.001	0.004	0.76	0.007	0.002	0.007
CRP (0 h)	0.007	0.008	0.34	0.018	0.004	0.0001
MCP1 (0 h)	− 0.001	0.002	0.55	− 0.0003	0.001	0.76
IL2SRA(0 h)	− 0.006	0.002	0.02	0.002	0.001	0.09
Adiponectin (0 h)	− 0.003	0.004	0.46	− 0.003	0.002	0.24
TNFα (0 h)	− 0.0008	0.003	0.80	0.007	0.002	0.0001

Since the models include a quadratic term the effect of a one unit change in x on y is not constant
EEAA extrinsic epigenetic age acceleration, 0 h fasting time point, 6 h postprandial time point, CRP C-reactive protein, HDL high-density lipoprotein, IL2SRA interleukin-2 soluble receptor alpha, IL6 interleukin-6, LDL low-density lipoprotein, MCP1 monocyte chemotactic protein 1, SE standard error, TC total cholesterol, TG triglyceride, TNFa tumor necrosis factor alpha
*Model is adjusted for adjusted for age, study site, sex, fasting lipid level (if applicable), estimated T cell type percentages, epigenetic age acceleration estimate squared (i.e., a quadratic polynomial), current smoking status, current drinking status, and a random effect of family relationship
#Model is adjusted for adjusted for age, study site, sex, fasting lipid level (if applicable), epigenetic age acceleration estimate squared (i.e., a quadratic polynomial), current smoking status, current drinking status, and a random effect of family relationship

ISSN: 1868-7083