Skip to main content
Fig. 5 | Clinical Epigenetics

Fig. 5

From: MEST mediates the impact of prenatal bisphenol A exposure on long-term body weight development

Fig. 5

In vitro adipocyte differentiation from human MSCs: exposure to BPA (10 μM, 50 μM) compared to solvent control (EtOH 0.05%). a Real-time monitoring of cell differentiation (xCELLigence: normalized cell index) over a 17-day period (mean ± SD, n = 4). b Quantification of Oil Red O stained area (mean ± SD, n ≥ 20 from one experiment). c Exemplary histological Oil Red O staining of adipocytes (black bar = 100 μm). d qPCR data of genes involved in adipogenesis (n ≥ 3) normalized to EtOH control (Lep = leptin, LPL = lipoprotein lipase, PPARγ = peroxisome proliferator activated receptor gamma, IRS2 = insulin receptor substrate 2, FASN = fatty acid synthase, SREBF1 = sterol receptor element binding factor 1, ESR1 = estrogen receptor alpha). e Targeted MassARRAY analysis of MEST promoter methylation, shown are the measurement of the single CpG cg17580798 covered by the amplicon (gray bars, n = 3) and the mean of the MassARRAY amplicon (black bars). f qPCR data of MEST (n ≥ 3, relative to EtOH control); *p < 0.05, **p < 0.01, ***p < 0.001 from Student’s t test/ANOVA

Back to article page