Fig. 2

450k array-wide differential DNAm volcano plots in high-risk MTHFR 677 and high-risk 1298 placentas. Differential methylation was determined using a linear model with the MTHFR group as the main effect and fetal sex and gestational age included as covariates. The magnitude of difference (Δβ) between risk groups and reference group is depicted on the x-axis, and the significance of the comparison (−log10(adjusted p value)) is on the y-axis, for every CpG tested on the 450k array (N = 442,355). a Differential methylation results between high-risk 677 and reference placentas. b Differential methylation results between high-risk 1298 and reference placentas. Neither comparison identified any CpG sites differentially methylated between the high-risk MTHFR placentas and reference placentas. 450k array Illumina Infinium HumanMethylation450 BeadChip, FDR false discovery rate