Fig. 4
RNA sequencing results upon UHRF1 knockdown in HUH6 cells. Log2 fold change of a known UHRF1 target genes, c genes related to the LIN28B pathway, and d genes highly expressed in the adverse C2 gene signature. b Significant enrichment of up- or downregulated genes within the indicated gene ontology and hallmark gene sets. Enrichment is indicated by the corresponding representation factor of each gene set. e Scratch assay. Representative images of HUH6 cells transfected with siRNA against UHRF1 migrating into a cell-free space at 0, 48, and 96 h compared to negative control (siNTC). Quantitative evaluation of scratch closure shows the mean values of two independent experiments