The new 6q27 tumor suppressor DACT2, frequently silenced by CpG methylation, sensitizes nasopharyngeal cancer cells to paclitaxel and 5-FU toxicity via β-catenin/Cdc25c signaling and G2/M arrest

Table 1 List of primers used in this study

PCR	Primer	Sequence (5′-3′)	Product size (bp)	PCR cycles	Annealing temperature (°C)
RT-PCR	DACT2F	AGCCGTGGGGCACATTCTG	173	32	55
	DACT2R	CCAGGTCCTGCCGATACTTG		32
	β-actinF	TCCTGTGGCATCCACGAAACT	315	23	55
	β-actinR	GAAGCATTTGCGGTGGACGAT		23
qRT-PCR	CyclinB1F	TCTGGATAATGGTGAATGGACA	157		60
	CyclinB1R	CGATGTGGCATACTTGTTCTTG
	MMP2F	CATACAGGATCATTGGCTACACAC	99		60
	MMP2R	GCAGTGGGGTCACATCGCT
	MMP9F	CCTGGAGACCTGAGAACCAATC	79		60
	MMP9R	CCACCCGAGTGTAACCATAGC
	Cdc25cF	GATGTCCCTAGAACTCCAGTG	120		60
	Cdc25cR	AGTTATCTCCCCACTGCTAAGA
	β-actinF1	GTCTTCCCCTCCATCGTG	113		60
	β-actinR1	AGGGTGAGGATGCCTCTCTT
MSP	DACT2m1	CGTGTAGATTTCGTTTTTCGC	200	40	60
	DACT2m2	CCGAAAATCCGCCCGACG		40
	DACT2u1	TGTGTGTAGATTTTGTTTTTTGT	203	40	58
	DACT2U2	CCCCAAAAATCCACCCAACA		40
BGS	DACT2BGS1	GGTTATAGATTTTAGTTTATTTTGG	249	40	60
BGS	DACT2BGS2	CTACAACTCCTACAACCCC		40

ISSN: 1868-7083