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Fig. 5 | Clinical Epigenetics

Fig. 5

From: A robust internal control for high-precision DNA methylation analyses by droplet digital PCR

Fig. 5

The 4Plex acts as a template-loading control that prevents erroneous scoring of template-negative samples. Upper panel: amplification of VIM in a selection of cell lines (blue, positive droplets; black/gray, negative droplets). Lower panel: amplification of the 4Plex in the same cell lines (green, positive droplets; black/gray, negative droplets). Water is used as a non-template control (NTC)

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