Fig. 3
From: A robust internal control for high-precision DNA methylation analyses by droplet digital PCR
The 4Plex has minor effects on the non-normalized concentrations of the target. a Non-normalized CDO1 concentrations from a singleplex experiment, and from an experiment with the 4Plex, are plotted. Upper plot: x-axis: the 34 colorectal cancer cell lines. y-axis: non-normalized CDO1 concentrations in methylated copies/microliter. Results from the singleplex experiment are shown in red and from the experiment with the 4Plex in blue. Lower panel: x-axis: non-normalized CDO1 concentrations from a singleplex experiment. y-axis: non-normalized CDO1 concentrations from an experiment with the 4Plex. Each dot represents one cell line. b The same plots as in a, but for SEPT9