Fig. 5

Flow cytometry analysis demonstrating expression of EGFR on the cell surface of various cell lines. The curve shaded gray represents binding of rat monoclonal antibody to human EGFR tagged with phycoerythrin (PE). The curve without shading (white) represents binding of an isotype control, which in the case was a PE-tagged monoclonal rat IgG2a kappa. The vertically written numbers next to each peak represent median fluorescence intensity for isotype control and anti-EGFR PE. For all cell lines tested (UMUC1, UMUC5, Scaber, HT1376, SW780, and 253 J), there was substantial EGFR expression compared with isotype control