Identification of reliable biomarkers of human papillomavirus 16 methylation in cervical lesions based on integration status using high-resolution melting analysis

Table 1 Fifteen pairs of primers were designed using Primer Premier 6 software based on previously reported interrupted sites in the HPV 16 genome

Primer	Sequence (5′–3′)	Size (bp)
E2BSs	F-ccgaccccttatattatggaatctt
E2BSsa	B-cagatgtctttgcttttcttcaggaca	1461
E1-1	F-atatcagatgacgagaacgaaaatga
E1-1a	B-gatacaggtgaagatttggtagattttatagta	2489
E1-2	F-gaaacaccatgtagtcagtatagtg
E1-2a	B-agtgggggtggtagcagtcagtatagtagtggaag	2126
E1-3	F-gaattatcacagatggtacaatg
E1-3a	B-cgataatgacatagtagacgatagtga	1521
E2-1	F-gtaaaacataaaagtgcaattg
E2-1a	B-acttacatatgatagtgaatgtcaacg	763
E2-2	F-agttgttgcacagagactccgt
E2-2a	B-agtgctccaatcctcactgcag	975
E2-3	F-ctttggtgcccaaggcgacg
E2-3a	B-tatgggtcgcggcggagtggttggcc	2553
E2-4	F-tgatgtgtatgtagacacagacaaaagcagcggacgta
E2-4a	B-ataggcagacacacaaaagcaca	450
L2-1	F-tagaattacaaactataacacc
L2-1a	B-tctacatatacaaccacttcccatgc	779
L2-2	F-agtacgcctagaggttaatgctg
L2-2a	B-ctggcctatgtaaagcaactata	121
L2-3	F-ataatgtcaggtggac
L2-3a	B-catgttttataaagttgggtggc	821
L1-1	F-tccatagatatacattctct
L1-1a	B-ctgtgtcatccaatttatttaata	791
L1-2	F-caagcaattgcctgggatg
L1-2a	B-ctataagtatcttctagtgtgc	740
L1-3	F-gcaaaggatccccatgtaacaatg
L1-3a	B-caggtgattgtccaccattagagttaat	804
E6-E7	F-cttatattatggaatctttgc
E6-E7a	B-gtccagatgtctttgcttttc	1522

Primer sets for DIPS-PCR of HPV16 integration analysis. Primers without subscript are used for the first linear PCR and with a subscript are used for the second exponential PCR

ISSN: 1868-7083