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Table 1 Fifteen pairs of primers were designed using Primer Premier 6 software based on previously reported interrupted sites in the HPV 16 genome

From: Identification of reliable biomarkers of human papillomavirus 16 methylation in cervical lesions based on integration status using high-resolution melting analysis

Primer Sequence (5′–3′) Size (bp)
E2BSs F-ccgaccccttatattatggaatctt  
E2BSsa B-cagatgtctttgcttttcttcaggaca 1461
E1-1 F-atatcagatgacgagaacgaaaatga  
E1-1a B-gatacaggtgaagatttggtagattttatagta 2489
E1-2 F-gaaacaccatgtagtcagtatagtg  
E1-2a B-agtgggggtggtagcagtcagtatagtagtggaag 2126
E1-3 F-gaattatcacagatggtacaatg  
E1-3a B-cgataatgacatagtagacgatagtga 1521
E2-1 F-gtaaaacataaaagtgcaattg  
E2-1a B-acttacatatgatagtgaatgtcaacg 763
E2-2 F-agttgttgcacagagactccgt  
E2-2a B-agtgctccaatcctcactgcag 975
E2-3 F-ctttggtgcccaaggcgacg  
E2-3a B-tatgggtcgcggcggagtggttggcc 2553
E2-4 F-tgatgtgtatgtagacacagacaaaagcagcggacgta  
E2-4a B-ataggcagacacacaaaagcaca 450
L2-1 F-tagaattacaaactataacacc  
L2-1a B-tctacatatacaaccacttcccatgc 779
L2-2 F-agtacgcctagaggttaatgctg  
L2-2a B-ctggcctatgtaaagcaactata 121
L2-3 F-ataatgtcaggtggac  
L2-3a B-catgttttataaagttgggtggc 821
L1-1 F-tccatagatatacattctct  
L1-1a B-ctgtgtcatccaatttatttaata 791
L1-2 F-caagcaattgcctgggatg  
L1-2a B-ctataagtatcttctagtgtgc 740
L1-3 F-gcaaaggatccccatgtaacaatg  
L1-3a B-caggtgattgtccaccattagagttaat 804
E6-E7 F-cttatattatggaatctttgc  
E6-E7a B-gtccagatgtctttgcttttc 1522
  1. Primer sets for DIPS-PCR of HPV16 integration analysis. Primers without subscript are used for the first linear PCR and with a subscript are used for the second exponential PCR
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Clinical Epigenetics

ISSN: 1868-7083

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