Fig. 5

MassARRAY quantitative methylation analysis of promoter CpG sites of target genes. a The schematic diagram of amplicon in the promoter region of CD9 (up). The mean methylation levels for each CpG site of the CD9 promoter (down). The correlations between CD9 mRNA expression level and the average CpG site methylation level in the promoter of CD9 (right). b The schematic diagram of amplicon in the promoter region of NR4A1 (up). The mean methylation levels for each CpG site of the NR4A1 promoter (down). c The schematic diagram of amplicon in the promoter region of EDN2 (up). The mean methylation levels for each CpG site of the EDN2 promoter (down). d The schematic diagram of amplicon in the promoter region of LIF (up). The mean methylation levels for each CpG site of the LIF promoter (down). The associations between average methylation level of LIF and its expression (right). e The schematic diagram of amplicon in the promoter region of BNIP3 (up). The mean methylation levels for each CpG site of the BNIP3 promoter (down). f The schematic diagram of amplicon in the promoter region of SPP1 (up). The mean methylation levels for each CpG site of the SPP1 promoter amplified by primer 2 (down). Binding sties for each of the forward and reverse primers are shown as arrows below the diagram. Values are mean ± SE. Thirty-eight GC genomic DNA from controls and 47 GC genomic DNA from PCOS women were analyzed. P value is determined by Mann-Whitney U test. * represents P < 0.05, ** represents P < 0.01