Table 1 Treatment doses for single and combined treatment. Eight UCCs, two immortalized benign urothelial cells (TERT-NHUC, HBLAK), primary urothelial cells (NHUC), and HEK-293 cells were treated with JQ1 at a fixed range of concentrations. Cell viability was measured by MTT- assay 72 h later. IC₅₀ values for each cell line were determined (upper part). Cell viability results were used according to the Chou-Talalay method to determine doses for the combination treatment with synergistic effects for each investigated cell line. The respective doses were applied for further functional characterization for 48 h (lower part)

From: Combined inhibition of BET proteins and class I HDACs synergistically induces apoptosis in urothelial carcinoma cell lines