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Fig. 3 | Clinical Epigenetics

Fig. 3

From: TFF1 hypermethylation and decreased expression in esophageal squamous cell carcinoma and histologically normal tumor surrounding esophageal cells

Fig. 3

Promoter methylation contributes to the downregulation of TFF1 mRNA and protein levels in vitro. a Cell viability, assessed by trypan blue staining, of TE-1 and TE-13 cells after treatment with DMSO or 2.5 μM of decitabine for 72 h. b TFF1 promoter methylation status, assessed by pyrosequencing, in TE-1 and TE-13cells after treatment with DMSO or 2.5 μM of decitabine for 72 h. c TFF1 mRNA expression levels, assessed by RT-qPCR, in TE-1 and TE-13cells after treatment with DMSO or 2.5 μM of decitabine for 72 h. d TFF1 protein expression, assessed by western blot, in TE-1 and TE-13cells after treatment with DMSO or 2.5 μM of decitabine for 72 h.*p < 0.05

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