Fig. 3
From: Feasibility of quantifying SDC2 methylation in stool DNA for early detection of colorectal cancer
Methylation status of SDC2 in stool DNA by meSDC2 LTE-qMSP test. a meSDC2 LTE-qMSP test was performed in stool DNA from CRC patients in varying stages, adenoma patients (Ade), and healthy normal subjects (N). Distribution of the relative level of SDC2 methylation was expressed in CT values as 40-CT for each sample. A higher 40-CT represents a higher methylation level of SDC2. It is represented as 0, if the SDC2 CT was not detected. Methylation status of the SDC2 gene is plotted as box and whisker plots. The difference in methylation level of SDC2 was statistically significant at **P < 0.01 and *P < 0.05 calculated by Kruskal-Wallis test in CRC patients vs. healthy normal subjects and adenoma patients vs. healthy normal subjects, respectively. b ROC curve was plotted for CRC patients vs. healthy normal subjects. The cutoff value for methylation-positive and AUC are indicated in the plot