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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Feasibility of quantifying SDC2 methylation in stool DNA for early detection of colorectal cancer

Fig. 2

Schematic diagram and analytic performance of meSDC2 LTE-qMSP. a Outline of meSDC2 LTE-qMSP. LTE was first performed using two primers: SDC2 and COL2A1 antisense primers attached to a universal primer to enrich methylated SDC2 DNA, and consecutive duplex real-time PCR was carried out using methylation-specific SDC2 sense primer, COL2A1-specific sense primer, universal primer, and probes. b The LoD of meSDC2 LTE-qMSP was compared with that of meSDC2-qMSP. Different amounts of HCT116 genomic DNA were diluted in unmethylated genomic DNA in total 20 ng of genomic DNA

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