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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Comparison of quantification algorithms for circulating cell-free DNA methylation biomarkers in blood plasma from cancer patients

Fig. 3

Training cohort. Kaplan-Meier analysis of overall survival in HNSCC patients (n = 129). Patients are stratified according to SHOX2 and SEPT9 plasma methylation levels. Plasma methylation levels were quantified using relative, absolute quantification, and quasi-digital PCR and dichotomized based on cutoffs that resulted in specificities and sensitivities as seen in Figs. 1 and 2. Cutoff values for positive (above cutoff) and negative (below cutoff) classification: SEPT9, SHOX2, and mean SEPT9/SHOX2 . Cutoffs for relative quantification were SEPT9 = 0.055%, SHOX2 = 0.281%, and mean SEPT9/SHOX2  = 0.118%; for absolute quantification SEPT9 = 4.68 pg, SHOX2 = 22.7 pg, and mean SEPT9/SHOX2  = 14.3 pg; for quasi-digital PCR for SEPT9 > 2, for SHOX2 > 4 and for mean SEPT9/SHOX2  > 2 positive PCR reactions

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Clinical Epigenetics

ISSN: 1868-7083

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