Fig. 1

VGF is differentially methylated and expressed in tumor-free lung tissues and NSCLC tissues. Normalized methylation analysis of CpG loci for VGF present on the HumanMethylation450K BeadChip with tumor samples indicated in red and matched tumor-free controls indicated in green (heatmap: yellow high, blue low DNA methylation values; mean DNA methylation = 0) (a). Relative gene expression level of VGF as quantile-normalized expression values of tumor-free lungs and matched tumors depicting the median with the 95% confidence interval as error bars. Paired two-sided T test with p ≤ 0.05 (*) regarded significant was used for statistical analysis (b). Exemplary results from IHC analysis showing VGF expression on protein level in a selected patient tissue (c)