Table 4 Primers used for real-time quantitative PCR
Symbol | Gene description | Forward primer | Reverse primer |
|---|---|---|---|
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | TGCCCTCAACGACCACTTT | GGTCCAGGGGTCTTACTCCTT |
DNMT1 | DNA methyltransferase 1 | CCTGTACCGAGTTGGTGATGGT | CCTTCCGTGGGCGTTTC |
DNMT3A | DNA methyltransferase 3 alpha | CTCCTGTGGGAGCCTCAATGTTACC | CAGTTCTTGCAGTTTTGGCACATTCC |
DNMT3B | DNA methyltransferase 3 beta | ACCACCTGCTGAATTACTCACGC | GATGGCATCAATCATCACTGGATT |
TET1 | tet methylcytosine dioxygenase 1 | AATGGAAGCACTGTGGTTTG | ACATGGAGCTGCTCATCTTG |
TET2 | tet methylcytosine dioxygenase 2 | AATGGCAGCACATTGGTATG | AGCTTCCACACTCCCAAACT |
TET3 | tet methylcytosine dioxygenase 3 | TTGCGTCGAACAAATAGTGG | CCCGTGTAGATGACCTTCTC |