Table 2 Analytical sensitivity in a background of unmethylated DNA, determined in three independent experiments, each in triplicate
From: Performance of a methylation specific real-time PCR assay as a triage test for HPV-positive women
Proportion of methylated DNA in 20 ng input DNA | Amount of methylated DNA | ASTN1 | DLX1 | ITGA4 | RXFP3 | SOX17 | ZNF671 | ACHE | IDS |
|---|---|---|---|---|---|---|---|---|---|
10% | 2 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 |
5% | 1 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 |
2% | 0.4 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 |
1% | 0.2 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 8/9 |
0.5% | 0.1 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 8/9 |
0.2% | 0.04 ng | 9/9 | 9/9 | 9/9 | 9/9 | 8/9 | 9/9 | 9/9 | 7/9 |