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Table 1 Primer sequences and PCR conditions

From: Methylation of ZNF331 is an independent prognostic marker of colorectal cancer and promotes colorectal cancer growth

Gene Forward primer Reverse primer Amplicon size (bp) Annealing temperature (°C) PCR cycles
ZNF331 U 5′-TTTTAAGGTAGGATGTTTTTAGGGTTGT-3′ 5′-ACAACTCTACACAACACAAATAAAACCA-3′ 147 60 35
ZNF331 M 5′-TAAGGTAGGACGTTTTTAGGGTCGC-3′ 5′-AACTCTACACGACGCAAATAAAACCG-3′ 142 60 35
CANA1G U 5′-TTTTTTTGTTTTGTGTTTAGGTTTT-3′ 5′-CCCTCTCAAAACAACTTCACCA-3′ 71 64 35
CANA1G M 5′-TCGTTTCGCGTTTAGGTTTC-3′ 5′-CTCGAAACGACTTCGCCG-3′ 62 64 35
IGF2 U 5′-GGAGTGGTTTTGGTGTTGTTATT-3′ 5′-CCCAACTCAATTTAAACCAACA-3′ 90 66 35
IGF2 M 5′-GCGGTTTCGGTGTCGTTATC-3′ 5′-CCAACTCGATTTAAACCGACG-3′ 86 68 30
RUNX3 U 5′-GTTGGTGGATTATGTAGGTGAGTTT-3′ 5′-ACTCACCTTAAAAACAACAAACAACA-3′ 115 66 35
RUNX3 M 5′-GCGGATTACGTAGGCGAGTTC-3′ 5′-ACCTTAAAAACGACGAACAACG-3′ 107 66 35
MLH1 U 5′-TTTTGATGTAGATGTTTTATTAGGGTTGT-3′ 5′-ACCACCTCATCATAACTACCCACA-3′ 124 60 35
MLH1 M 5′-ACGTAGACGTTTTATTAGGGTCGC-3′ 5′-CCTCATCGTAACTACCCGCG-3′ 115 60 35
KRAS exon2 5′-AAGGTGAGTTTGTATTAAAAGGTACTGG-3′ 5′-TGGTCCTGCACCAGTAATATGC-3′ 264 58 35
BRAF- 600E 5′-TCATAATGCTTGCTCTGATAGGA-3′ 5′-CTTTCTAGTAACTCAGCAGC-3′ 251 60 35