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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Methylation of ZNF331 is an independent prognostic marker of colorectal cancer and promotes colorectal cancer growth

Fig. 1

Expression and methylation status of ZNF331 in CRC cells. a Expression of ZNF331 was detected by semi-quantitative RT-PCR in CRC cells. H2O: double-distilled water, negative control. GAPDH: internal control. DAC 5-aza-2′-dexycytidine, (−) absence of DAC, (+) presence of DAC. b Relative mRNA expression level of ZNF331 before and after treatment with DAC was detected by real-time quantitative RT-PCR. (***p < 0.01). c MSP results in CRC cell lines. IVD in vitro methylated DNA (methylation control), NL lymphocyte DNA (unmethylation control), U unmethylated alleles, M methylated alleles. d Bisulfite sequencing results: double-headed arrow indicates the region of the MSP product. Filled circles: methylated CpG sites; open circles: unmethylated CpG sites. TSS: transcription start site

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