Fig. 6
From: Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure
Effects of PAE on the fractional synthesis of H3K9 and its modified peptides in frontal cortex and hypothalamus of rat brain. We measured the incorporation of 2H in the histone H3 peptide H3K9 (kSTGGKAPR) and its di-methylated (H3K9me2), tri-methylated (H3K9me3), and acetylated (H3K9ac) isoforms on lysine 9, in frontal cortex (a) and hypothalamus (b) of 6-day-old rat pups following a 5-day alcohol feeding and administration of 2H2O. Histone Fractional Synthesis Rate (FSR) was calculated and data are shown as mean ± SE, n = 4 per group, ***p < 0.001, AF vs PF, #p < 0.05, modified vs unmodified peptides, a p < 0.05, H3K9me3 vs H3K9 and H3K9me2