Epigenome-wide association study of asthma and wheeze in childhood and adolescence

Clinical Epigenetics

Table 3 Results of epigenome-wide association studies (EWAS), where methylation is the outcome and current asthma at 7.5 years, current wheeze at 7.5 years, current asthma at 16.5 years, ever asthma at 16.5 years and ever wheeze at 16.5 years are the exposures

Methylation (outcome)	Asthma/wheeze (exposure)	N [cases/controls]	Cell adjustment	FDR hits [Bonferroni]	Lambda
7.5 years	Current asthma at 7.5 years	781 [149/632]	No cells	411 [67]	1.08
			Basic cells	302 [43]	1.01
			Detailed cells	0 [0]	0.97
	Wheeze at 7.5 years	853 [111/742]	No cells	611 [105]	1.14
			Basic cells	445 [78]	1.08
			Detailed cells	0 [0]	1.00
16.5 years	Ever asthma at 16.5 years	748 [194/554]	No cells	0 [0]	1.09
			Basic cells	0 [0]	1.05
			Detailed cells	0 [0]	1.01
	Ever wheeze at 16.5 years	758 [204/554]	No cells	48 [9]	1.02
			Basic cells	32 [9]	1.05
			Detailed cells	0 [0]	1.01
	Current asthma at 16.5 years	611 [184/427]	No cells	2 [2]	1.01
			Basic cells	2 [2]	1.05
			Detailed cells	2 [1]	0.99

All models are adjusted for confounders and technical covariates. Stepwise adjustments for estimated cell counts were made (where basic cells include CD8T, CD4T, NK, monocytes, B cells and granulocytes and detailed cells include eosinophils and neutrophils instead of granulocytes)
FDR-adjusted P value and Bonferroni hits use a cut-off of < 0.05. Lambda based on P values of all probes without exclusions

ISSN: 1868-7083