Fig. 4

Increased expression levels of miR-335 inhibit cell invasion, cell migration, anchorage-independent cell growth, stemness properties, and cell viability in Hs 746T cells. a Comparison of invasive properties of AGS and Hs 746T cells. b Representative images for Hs 746T cells transfected with control NC/miR-335 mimic in invasion assay. c Hs 746T cells transfected with control NC/miR-335 mimic in migration assay. d Hs 746T cells transfected with control NC/miR-335 mimic in soft agar assay. Formation of colonies > 50 μm in diameter was scored at 2 weeks. e Hs 746T cells transfected with control NC/miR-335 mimic in spheroid formation assay. f Viability of Hs 746T cells transfected with control NC/miR-335 mimic in MTT assay. Results represent the means of three independent experiments, bars indicate SD. *р < 0.05, **p < 0.01, ***p < 0.001, ****р < 0.0001. WT, wild-type