Fig. 1
HOXD10 expression and methylation status in human HCC cells. a Semi-quantitative RT-PCR shows HOXD10 expression levels in HCC cell lines. HBXF344, SNU182, SNU387, SNU449, SNU475, SMMC7721, BEL7402, Huh1, Huh7, LM3, PLC/PRF/5, HepG2, and QGY7703 are HCC cell lines. 5-AZA: 5-aza-2′-deoxycytidine; GAPDH: internal control of RT-PCR; (−): absence of 5-AZA; (+): presence of 5-AZA. b MSP results of HOXD10 in HCC cell lines. U: unmethylated alleles; M: methylated alleles; IVD: in vitro methylated DNA, serves as methylation control; NL: normal peripheral lymphocytes DNA, serves as unmethylated control; H2O: double distilled water. c BSSQ results of HOXD10 in SMMC7721, Huh7 and Huh1 cells. Double-headed arrow: MSP PCR product size was 100 bp and bisulfite sequencing focused on a 254 bp region of the CpG island (from − 375 to − 122) around the HOXD10 transcription start site. Filled circles: methylated CpG sites, open circles: unmethylated CpG sites. TSS: transcription start site