Fig. 5

MBD4 regulates the CD70 promoter activity by changing promoter methylation status. a Relative firefly luciferase activity in HEK293T cells co-transfected with an empty vector (pEZ-M13) or an MBD4-expressing vector (pEZ-M13 MBD4), together with luciferase reporter constructs containing the CD70 gene promoter. The luciferase activity was decreased in HEK293 T cells transfected with MBD4 expression vector compared with empty control vector, suggesting MBD4 represses the activity of CD70 promoter. b The result of R-MeDIP-qPCR showed that the methylation level of the inserted CD70 promoter in luciferase reporter vector was increased in HEK293T cells transfected with MBD4 expression vector compared with empty control vector. Three independent experiments were performed (**P < 0.01, *P < 0.05)