Fig. 3

Upregulation of MBD4 represses CD70 expression in SLE CD4⁺ T cells. a The western blot results show the increased expression level of MBD4 protein in CD4⁺ T cells after 48 h post-transfection of MBD4 expression plasmid. b The mRNA level of CD70 was decreased in SLE CD4⁺ T cells transfected with the MBD4 plasmid compared with the negative control. c, d Transfected cells were stained with anti-CD4-FITC antibody and anti-CD70-PE antibody and analyzed with flow cytometry (c). The percentages of CD70-staining CD4⁺ T cells are shown in (d). All data represent the mean of three independent experiments per group (*P < 0.05)