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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Down-regulation of MBD4 contributes to hypomethylation and overexpression of CD70 in CD4+ T cells in systemic lupus erythematosus

Fig. 2

Knockdown of MBD4 upregulates CD70 expression in normal CD4+ T cells. a Western blot showing the decreased expression of MBD4 protein in CD4+ T cells 48 h post-transfection of MBD4 siRNA. b The mRNA level of CD70 was significantly upregulated in CD4+ T cells transfected with MBD4 siRNA compared with negative control. c, d Transfected cells were stained with anti-CD4-FITC antibody and anti-CD70-PE antibody and analyzed using flow cytometry (c). The percentages of CD70-staining CD4+ T cells are shown in (d). We first gated on CD4+ T cells and then defined CD70-positive T cells (c). All data represent the mean of three independent experiments per group (**P < 0.01, *P < 0.05)

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