Fig. 2

Knockdown of MBD4 upregulates CD70 expression in normal CD4⁺ T cells. a Western blot showing the decreased expression of MBD4 protein in CD4⁺ T cells 48 h post-transfection of MBD4 siRNA. b The mRNA level of CD70 was significantly upregulated in CD4⁺ T cells transfected with MBD4 siRNA compared with negative control. c, d Transfected cells were stained with anti-CD4-FITC antibody and anti-CD70-PE antibody and analyzed using flow cytometry (c). The percentages of CD70-staining CD4⁺ T cells are shown in (d). We first gated on CD4⁺ T cells and then defined CD70-positive T cells (c). All data represent the mean of three independent experiments per group (**P < 0.01, *P < 0.05)