Fig. 1
From: Epigenetic response of imprinted domains during carcinogenesis
Targeting oncogenic KrasG12D to mouse T cells and epithelial cells. a Structure of the conditional LSL-Kras G12D allele. Once Cre removes the transcriptional stop cassette, transcription of oncogenic KrasG12D proceeds. b Breeding scheme involving mouse lines LSL-Kras G12D and MMTV-Cre. The numbers in parentheses indicate how many pups were born in each litter, the red number indicates the average litter size, and the blue numbers indicate how many mutant mice were obtained out of the expected number based on Mendelian inheritance. c Gross image of a thymic T cell lymphoma that is markedly expanding the anterior mediastinum in a KrasG12D mouse. d Gross image of a squamous papilloma in and around the mouth of a KrasG12D mouse. e Histological image of a thymic T cell lymphoma in a KrasG12D mouse with neoplastic lymphoid cells completely effacing the normal architecture of the thymus and surrounding mediastinal soft tissue (200× magnification). f Histological image of a squamous papilloma in a KrasG12D mouse showing the proliferated epithelium forming multiple finger-like projections with maintenance of the tissue boundaries (100× magnification)