Fig. 3

Inhibitor effects on methylation status occur as early as 48 h post treatment. Left: Western Blot analysis of H3K27me3. Coomassie blue staining (shown below Western blot) utilized as loading control. Cells were harvested and subjected to Western Blot analysis of H3K27me3 in 24 h intervals from 24 to 168 h (1–7 days) following the addition of an inhibitor (0.5 μM GSK-343, 1.0 μM DZNeP, or 5.0 μM EPZ-5687) at hour 0. Right: Quantification of Western blot to left of graph based on fold differences to DMSO-treated cells