Fig. 4
From: LSD1 dual function in mediating epigenetic corruption of the vitamin D signaling in prostate cancer
ChIP analysis of BC1A cells stably transfected with shLSD1 lentiviral vector and treated for 24 h with 100 nM 1,25-D3. Each bar indicates the percentage of binding relative to INPUT and represents the mean of at least three biological replicates with SEM. The columns indicate, from left to right, shCTR + Veh, shCTR + 1,25-D3, shLSD1 + Veh, and shLSD1 + 1,25-D3. The basal levels of the following protein/histone marks were evaluated, from left to right, in each graph: IgG, VDR, DNMT1, H3K4me2, and H3K9Ac. The regions analyzed were a Cdkn1a TSS, b Cdkn1a VDRE, c E2f1 TSS, d Cyp24a1 TSS, and e S100g TSS. IgG was used as a control for non-specific binding/enrichment. Statistical significance was calculated using one-way ANOVA and Tukey post hoc correction (***p < 0.001, **p < 0.01, *p < 0.05)