Fig. 5

Partial reversion of TGF-β1-induced EMT by epigenetic inhibitors in H358 cells. a Protocol: Cells were treated simultaneously with TGF-β1 (10 ng/ml), EPZ5676 (1 μM), SGC0946 (5 μM), or PFI-1 (5 μM) for 48 h and with SAHA (5 μM) for the last 12 h. b mRNA expression was measured by RT-qPCR and normalized to GAPDH mRNA for control cells (white bars) and TGF-β1 treated cells (black bars). The graph corresponds to the mean ± SD of two independent experiments with PCR in duplicate. *p < 0.5, **p < 0.01, ***p < 0.001, ****p < 0.0001 by a two-way ANOVA test. c Proteins were analyzed by immunoblotting of total cell lysates. Actin was used as a loading control. The apparent molecular weights (kDa) are indicated on the right of the panel