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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Tri-methylation of H3K79 is decreased in TGF-β1-induced epithelial-to-mesenchymal transition in lung cancer

Fig. 1

TGF-β1 induces EMT in NSCLC cell lines. Expression of epithelial and mesenchymal markers was determined by mRNA and protein analysis of A549 (white bars) and H358 (black bars) cells cultured with TGF-β1 (10 ng/ml) for the indicated time (h). a mRNA expression was measured by RT-qPCR and normalized to GAPDH mRNA level. The graph corresponds to the mean ± SD of two independent experiments with PCR in duplicate. *p < 0.5, **p < 0.01, ***p < 0.001 by a two-way ANOVA test. b Protein expression was determined by immunoblotting of the A549 and H358 total cell lysates. Actin was used as a loading control. The apparent molecular weights (kDa) are indicated on the right of the panel

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Clinical Epigenetics

ISSN: 1868-7083

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