Fig. 5

Chidamide posttranslationally but not transcriptionally inhibited the repair of IDA-induced DNA damage in CD34⁺CD38⁻ KG1α cells. CD34⁺CD38⁻ KG1α cells were incubated with 0.75 μM chidamide ±40 nM IDA for 24 and 48 h, followed by qRT-PCR to evaluate the gene expression of BRCA1, ATM, CHK1, and CHK2 (a), or western blot analysis to monitor the expression of p-BRCA1, p-ATM, p-CHK1, and p-CHK2 (b). β-actin was used as a loading control