Fig. 4

Chidamide increased IDA-induced DNA damage in CD34⁺CD38⁻ KG1α cells. CD34⁺CD38⁻ KG1α cells were treated with or without 0.75 μM of chidamide in combination with 40 nM IDA for 24 h. (a) Immunostaining was performed with γH2A.X and counterstained with DAPI. Each experiment was performed in triplicate. (b) The expression of γH2A.X was examined in the presence or absence of chidamide with or without IDA for 24 h followed by flow cytometry. Filled-area histograms represent medium treated cells, whereas color histograms represent different treated group, and the compiled results from three experiments are shown in (c). (d) The expression of γH2A.X and acetylation of histone 3 were examined in the presence or absence of chidamide with or without IDA for 24 and 48 h followed by western blot