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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Cooperative effect of chidamide and chemotherapeutic drugs induce apoptosis by DNA damage accumulation and repair defects in acute myeloid leukemia stem and progenitor cells

Fig. 2

Chidamide synergized IDA-, DNR-, or Ara-C-induced apoptosis in both CD34+CD38 KG1α cells and primary relapsed or refractory AML CD34+ cells. CD34+CD38 KG1α cells were exposed to the indicated concentrations of IDA, DNR, or Ara-C with or without 0.75 μM chidamide for 24, 48, and 72 h (a), with or without 0.5 or 0.75 μM chidamide for 72 h (b), after which flow cytometric analysis was performed to determine the percentage of Annexin V+ cells. Horizontal lines represent the mean ± S.D. from three independent experiments. (c) Primary CD34+ AML cells were exposed to the 20 nM IDA with or without 0.75 μM chidamide, after which apoptotic ratios were determined by Annexin V staining and flow cytometry. (d) Representative data for flow cytometric analysis of hCD34 and Annexin V/PI staining in primary cells after exposed (48 h) to 20 nM IDA with or without 0.75 μM chidamide. *P < 0.05

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