Fig. 5
Validation of the K36me1 increase in triple negative breast cancer samples by PAT-H-MS. a Heatmap display of the log2 of ratios obtained for differentially modified forms of the H3 27–40 peptides in 10 luminal A-like and 10 triple negative breast cancer samples, using the classical PAT-H-MS approach. L/H relative abundances ratios obtained with the super-SILAC strategy (light channel: breast cancer biopsy, heavy channel: spike-in super-SILAC standard) normalized over the average value across the samples are shown. Modified peptides significantly different in the two subtypes are indicated by asterisks. b Ratios obtained for the indicated peptides in the luminal A-like or triple negative breast cancer samples shown in A. Samples were compared by t test. Error bars represent standard error from ten patient samples. **p < 0.01, ***p < 0.001