Fig. 1From: Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancersSchematic representation of the two-step 5hmC labelling reaction. a First, T4 β-GT enzymatic glucosylation of 5hmC with UDP-6-N3-Glu is performed. b Next, click reaction between the N3 group and the fluorescently labelled alkyne DBCO-Cy5 is performed. This two-step reaction results in fluorescently labelled 5hmCBack to article page